In general SPR measurements, only liquid and gas can be analyzed. In our SPRi configuration systems, only liquids with refractive index comprised between 1.30 and 1.37 can be analyzed. These can be pure molecules or crude samples. The analyzed samples are usually biomolecules such as cells, bacteria, virus, polymers, antibodies, proteins, peptides, DNA, RNA, Aptamers, carbohydrates, organic small molecules, …
Typical molecular analysis:
No specific preparation is required even for complex mixtures such as serum, blood, cell extracts, or others. All solutions can be injected through the SPRi system without pretreatment or dilution.
The only essential thing to do is to dissolve or to dilute the analyte in the running buffer in order to avoid refractive index changes between the analyte solution and the running buffer.
Can solid samples be analyzed?
No, it is not possible to analyze solids with SPR or SPRi techniques. The analyte must be in solution. We can only study liquids with refractive index comprised between 1.30 and 1.37.
Can gas sample be analyzed?
From technical point of view yes we can analyze gas with SPR technique. In our configuration, gasses can’t be analyzed. We can only study liquids with refractive index comprised between 1.30 and 1.37.
Can we work in an anaerobic environment?
No, there is no mean to create an anaerobic environment in the flow cell.
Examples of applications concerned by SPRi.
Our flexible and easy-to-use systems allow a large range of applications to be explored.
Typical applications:
Introduction of the strengths and weaknesses of the techniques and how to compare with other techniques providing similar information.
SPRi is a label-free technology. SPRi doesn’t require either labeling of the samples or a revelation step with a secondary labeled molecule. By labeling we mean: fluorescence, radioactivity or dye.
The interest of SPRi, in comparison with more conventional label-based technologies like ELISA (Enzyme-Linked ImmunoSorbent Assay), lies in the real time measurement of the interaction, the low reagent consumption and the time saving.
Real time measurement gives access to the evaluation of the kinetic parameters of the interaction (association rate constant, dissociation rate constant and affinity/avidity). SPRi adds an imaging capacity to SPR. This feature gives access to the follow-up from two to several hundred interactions in parallel (this we call multiplexing).
To date, SPRi is the only commercially available technology gathering automation, label-free, real time measurement and imaging of up to several hundred interactions in parallel, with the possibility of affinity calculation for each of these interactions.
